High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst

High internal phase Pickering emulsion (Pickering HIPE) stabilized by enzyme-decorated metal-organic frameworks (MOFs) nanoparticles is developed for biphasic biocatalysts to enhance lipase catalysis and recycling. Specifically, enzyme decorated are prepared via ZIF-8 physisorption of a model Candida antarctica Lipase B (CALB), named ZIF-8@CALB, be both stabilizer catalytic sites. An oil-in-water (o/w) HIPE with oil/water volume ratio 3 could then fabricated homogenizing p-nitrophenyl palmitate (p-NPP) n-heptane solution into the ZIF-8@CALB aqueous dispersion. The biocatalytic hydrolysis p-NPP conducted just standing system at room temperature. achieves product conversion up 48.9% within 0.5 h, whereas containing free CALB only stable 6.8% same time. Moreover, ZIF@CALB recovered simple centrifugation 800 rpm, reused in next cycle. equilibrium rate keeps over 40% all 8 cycles, reflecting high efficiency recyclability HIPE. This study provides new opportunity designing Enzyme-MOFs-based interfacial biocatalyst practical applications.